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Biocolor 彈性蛋白檢測試劑盒（Fastin elastin Assay）
簡要描述：Biocolor 彈性蛋白檢測試劑盒Biocolor Fastin elastin Assay 利用染料結(jié)合的方法來定量檢測釋放到組織培養(yǎng)液或從生物材料中提取的彈性蛋白。
產(chǎn)品型號：F2000- 廠商性質(zhì)：代理商
- 更新時間：2025-10-25
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產(chǎn)品介紹
Biocolor 彈性蛋白檢測試劑盒
Biocolor Fastin elastin Assay
利用染料結(jié)合的方法來定量檢測釋放到組織培養(yǎng)液或從生物材料中提取的彈性蛋白。
應用范圍：樣本可來源于細胞培養(yǎng)基和含細胞外基質(zhì)成分的組織提取物（如彈性軟骨、主動脈、皮膚等），可檢測可溶性彈性蛋白原和不可溶性彈性蛋白（抽提后以可溶性彈性蛋白多肽的形式檢測）。
樣本要求：樣本體積5-100ul，包含5-75ug的彈性蛋白
實驗操作步驟
彈性蛋白的分離：用彈性蛋白沉淀劑處理樣本，使彈性蛋白沉淀下來。
彈性蛋白與染料結(jié)合：裝有沉淀的彈性蛋白管中，加染料和反應液，反應60min以上
彈性蛋白-染料復合物獲得：去掉未結(jié)合的染料
復合物染料的釋放：添加染料釋放劑，使彈性蛋白-染料復合物中的染料釋放出來
釋放出的染料檢測：繪制標準曲線，513nm或405nm處定量檢測
產(chǎn)品訂購
Product Code: F2000 110 assays
Product Code: F4000 440 assays
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